Thr308
WebPhospho-AKT1 (Thr308) Antibody (44-602G) in IHC (P) Immunohistochemistry analysis of AKT/PKB (pT308) showing staining in the cytoplasm and nucleus of paraffin-embedded … WebEnter the email address you signed up with and we'll email you a reset link.
Thr308
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WebImmunogen. AKT1 [p Thr308] Antibody (18F3.H11) was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT11 protein. (Uniprot: P31749) Modification. p Thr308. Specificity. This antibody is specific for human and mouse AKT protein phosphorylated at T308. WebAkt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy …
WebFeb 5, 2016 · Consistent with our findings, Akt Thr308 phosphorylation has been observed in other models where raptor has been downregulated or inactivated (rapamycin). 44, 45, 46 Thus, based on this premise, we suggest that probably association of raptor with 14-3-3 proteins could serve two major purposes: (1) inhibit mTORC1 function to trigger … WebMay 4, 2024 · Inhibitory interneurons are critical for maintaining the excitatory/inhibitory balance. During the development cortical interneurons originate from the ganglionic eminence and arrive at the dorsal cortex through two tangential migration routes. However, the mechanisms underlying the development of cortical interneurons remain unclear. 3 …
WebFeb 23, 2024 · MCF-7, T47D and HCC1500 had activating mutations in PIK3CA gene, while loss-of-activity PTEN mutations were detected in sensitive cell lines, including ZR-75-1, which showed no changes or minimal increase in the amount of p-AKT(Ser473/Thr308) and p-ERK1/2(Thr202/Tyr204) induced by everolimus compared to the resistant cell line T47D … WebAll lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution Lane 1 : HeLa cells Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution Predicted band size: 56 kDa 10% gel. Running conditions: 60v, 30min; 120v 60min
WebJun 6, 2014 · Phosphorylation of both Thr308 and Ser473 as well as all other possible residues phosphorylated in response to insulin were dependent on PI3K activity. AKT2 phosphorylation was much less responsive to insulin treatment than AKT1. Insulin markedly increased phosphorylation of Thr308 or Ser473 in AKT1 and to a lesser degree AKT2.
WebMore>> This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308). Less<< Anti-phospho-Akt (Thr308) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and … shipwreck escape 攻略Webhamster AKT 1/2/3 (Thr308) in cell lysates. Detection of rat AKT 1/2/3 (Thr308) is also expected. The InstantOne ELISA™ kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. quick recipes with few ingredientsWebMay 24, 2011 · The phosphorylation of Thr308 is a more reliable biomarker for the protein kinase activity of Akt in tumour samples than Ser473. Any evaluation of the link between … quick recovery auto belle chasse laWebPlasmids construction and DNA mutagenesis. AKT tagged with hemagglutinin (HA) was cloned into the pCI-Neo vector via the XhoI and SalI sites. Mutation of AKT at Thr308 or … shipwreck escape レビューWebPhospho-AKT1 (Thr308) Antibody (710122) in ICC/IF. Immunofluorescence analysis Phospho-AKT pThr308 was done on 70% confluent log phase NIH/3T3 cells treated with … quick recovery dsi gasWebOxymatrine treatment decreased the level of Aktp-Thr308 protein, with little change in the total Akt level (Figure 2C and D). Also, oxymatrine treatment increased the expression of caspase-3 and Bax significantly at protein and RNA … quick recovery high efficient water heaterWebApr 14, 2024 · Salt-inducible kinase 1 (SIK1), an AMPK-related kinase, regulates energy response-related physiopathologic processes, such as gluconeogenesis and lipid metabolism ().Recently, SIK1, not AMPK, has been increasingly identified as a major player in tumor suppressor liver kinase 1 (LKB1)-deficient NSCLC ().However, the precise roles and … quick recovery hills