Synaptophysin molecular weight
WebWestern blot of purified anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti ... WebThey are positive for low-weight cytokeratins (Cam5.2) and often, but not always, high-molecular-weight cytokeratins (AE1/3). CK20 is positive in up to 25% of GI NETs and CK7 …
Synaptophysin molecular weight
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WebSynaptophysin, a vesicular integral membrane protein, is specifically expressed in neuroendocrine tissues. According to cDNA cloning studies, it has a molecular weight of 33,300 Dalton, one potential N-glycosylation site at the vesicle inside, four major hydrophobic domains as well as a C-terminus containing approximately 90 amino acids. Web1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). ICC/IF Use at an assay dependent concentration. IHC-Fr Use at an assay dependent …
WebSynaptophysin is a 38 kDa membrane glycoprotein expressed in neuroendocrine cells and neurons involved in transmission via synaptic vehicles. ... the antibody labels a major … WebThey are positive for low-weight cytokeratins (Cam5.2) and often, but not always, high-molecular-weight cytokeratins (AE1/3). CK20 is positive in up to 25% of GI NETs and CK7 is positive in only 11% of cases. 281 The most commonly used antibodies to detect neuroendocrine differentiation are synaptophysin and chromogranin A.
WebJun 29, 2016 · Synaptophysin was detected at the expected molecular weight of 33 kDa. Protein levels of synaptophysin were significantly reduced in schizophrenia subjects compared with controls (−27.84%; t 36 ... WebSynaptophysin is an integral membrane glycoprotein with a molecular weight of 38 kD. It is reported to occur in presynaptic vesicles of neurons in brain, spinal cord, retina, in similar …
WebMay 25, 1990 · Synaptophysin, a major membrane protein of synaptic vesicles, ... In addition, chemical cross-linking of native synaptophysin demonstrates that a low molecular weight protein is specifically associated with synaptophysin complexes and is lost upon reduction of the intramolecular disulfide bonds.
Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. The gene is located on the short arm of X chromosome (Xp11.23-p11.22). It is 12,406 bases in length and lies on the minus strand. The encoded protein has 313 amino acids with a … See more The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in … See more This gene has been implicated in X linked intellectual disability. Using immunohistochemistry, synaptophysin can … See more • Kalina M, Lukinius A, Grimelius L, Höög A, Falkmer S (1991). "Ultrastructural localization of synaptophysin to the secretory granules of normal glucagon and insulin cells in … See more • List of human genes See more Synaptophysin has been shown to interact with AP1G1 and SIAH2. See more • Synaptophysin at the U.S. National Library of Medicine Medical Subject Headings (MeSH) See more product liability attorneys philadelphiaWebApr 13, 2024 · Synaptophysin, a presynaptic marker, showed a significant reduction on day 14 in the ipsilateral region compared with the contralateral side. Then, a decrease was observed until day 28, suggesting cofilin overactivation and formation of rods/aggregates might have resulted in synaptic loss and PSCI ( Figure 4 A,C). product liability attorneys seattleWebDetects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Flow Cyt (Intra) 1/20 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype … product liability attorneys njWebSynaptophysin is a glycoprotein that is an integral part of the neuroendocrine secretory granule membrane 268,273 and is recognized by monoclonal antibody (SY38) in a variety of neuroendocrine tumors. ... (Cam5.2) and often, but not always, high-molecular-weight cytokeratins (AE1/3). relatively fewerWebValidated for Western Blotting. Highly specific and rigorously validated in-house, Synaptophysin (7H12) Mouse Monoclonal Antibody ... 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended. Electrotransfer to nitrocellulose membrane . relatively few studiesWebsynaptophysin: Calculated molecular weight: 313 aa, 34 kDa: Observed molecular weight: 38-40 kDa: GenBank accession number: BC064550: Gene symbol: SYP: Gene ID (NCBI) … relatively few 意味WebThe Multisubunit Structure of Synaptophysin Vol. 265, No. 15, Issue of May 25, pp. 88694673, 1990 Printed in CJ. S. A. RELATIONSHIP ... physin demonstrates that a low molecular weight pro- tein is specifically associated with synaptophysin com- plexes and is lost upon reduction of the intramolecular disulfide bonds ... relatively few synonym