Plvx-shrna2-puro
WebThe pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with efficient RNA … WebApr 11, 2024 · The PFN2-OE cells were generated using pLVX-mCMV-ZsGreen-PGK-Puro vector, and PFN2-KD cells were infected with pLVX-shRNA2-Puro. The sequences of profiling 2 primer and profilin 2-shRNA are listed in Supplementary Table 2. HUVECs and RBMECs were transduced for 24 h with recombinant lentivirus and cultured for 72 h.
Plvx-shrna2-puro
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WebpLVX-puro (Search Vector Database) Vector type Lentiviral Selectable markers Puromycin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number High Copy Gene/Insert Gene/Insert name None Resource Information Articles Citing this Plasmid 7 References Terms and Licenses WebJul 1, 2010 · pLVX-Puro Vector Information . PT4002-5 Catalog No. 632164. pLVX-Puro Vector Map and Multiple Cloning Site (MCS). Description. pLVX-Puro is an HIV-1-based, …
WebpLVX-shRNA2-puro vector contained scramble shRNA or empty pLVX-puro vector transfection were used as negative controls. The primers for plasmid construction were listed in Table S1. The transfection eciency was determined by RT-PCR. CCK‑8 Assay A Cell Counting Kit-8 (CCK-8) assay kit (96,992; Sigma-Aldrich) was used to determine cell viability. WebOct 4, 2024 · Then, medium was replaced to allow the knockdown for 2 or more days. pLVX-shRNA2-Puro vectors contain a puromycin resistance site. Puromycin (4 mg/mL; Sigma) was added to the media to select for resistant cells and to generate stable target-deficient cell lines and Polybrene (1.75 mg/mL; Sigma) to enhance viral infection.
Web丁香通为您提供pug35序列pug35商品详情介绍：价格：￥1000 - 1980，货号：jh2089，cas号：无，品牌：jhbio，产地：国内，详见丁香通pug35序列pug35商品详情页； WebJan 30, 2024 · Suppression of IAPP expression by pLVX-ShRNA2-Puro-IAPP exerted opposite effects of increasing pro-apoptotic Bax and caspase-3 gene expression (Figures …
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WebpLVX-IRES-Puro. Lentiviral vector for bicistronic expression of a gene together with a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Open in SnapGene. born 1990 generationWeb首先将靶向沉默TOE1基因的shRNA序列插入慢病毒载体pLVX-shRNA2-Puro，构建敲低TOE1基因的慢病毒载体pLVX-shTOE1。然后通过PCR扩增TOE1基因序列克隆至慢病毒载体pCDH-CMV-MCS-EF1-Puro，构建TOE1基因过表达的慢病毒载体pCDH-TOE1。同时构建pEGFP-TOE1真核表达载体，转染人胃癌细胞SGC ... born 1991 chinese horoscopeWebJan 21, 2024 · In vitro analyses revealed that pLVX-shRNA-HIF-1α effectively inhibited the expression of HIF-1α and VEGF and led to the activation of p-65 and p-IКBɑ, as well as decreased proinflammatory cytokine expression in cell culture. born 1991 how old todayWebNov 2, 2024 · OriGene offers pre-made lentiviral shRNA plasmid kits containing 4 sequence-verified expression cassettes that target your gene of interest. Each lentiviral cassette contains three major functional elements: shRNA, a … born 1992 age todayWebpLVX-shRNA2 Vector Information . PT4052-5 Catalog No. 632179. pLVX-shRNA2 Vector Map and Multiple Cloning Site (MCS). Description. pLVX-shRNA2 is an HIV-1-based, … born 1991 what ageWebpLVX-Puro Sequence and Map pLVX-Puro Lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) … havelock nc city managerWebAug 14, 2015 · zt1248 pLVX-aMHC Puro addgene 慢病毒过表达载体. zt1249 pLVX-cas9 addgene 慢病毒过表达载体. zt1250 pLVX-cGFPI addgene 慢病毒过表达载体. zt1251 pLVX-cGFPII addgene 慢病毒过表达载体. zt1252 pLVX-CMV addgene 慢病毒过表达载体. zt1253 pLVX-EFGL addgene 慢病毒过表达载体 born 1992