Web10 x Column Buffer; 100 mM MgATP Stock; 100 mM MgGTP; 100 mM Sodium orthovanadate; 100mM Tris Buffer; 10x Tris Buffer; 150 mM Mg AMP-PNP; 1M dithiothreitol (DTT) 1M MgSO4; 20 x Energy Regeneration System; 2X Rapid Ligation Buffer; 3 M KI; 30 mM Mg GTP-g-S; ... PHEM (500 mls) 2x. WebOct 16, 2024 · We tested four fixatives, 3.7% formaldehyde (FA), 4% paraformaldehyde (PFA), 4% paraformaldehyde in the cytoskeletal buffer (PFA + CB) and 4% …
Primary Culture of Cornea-Limbal Epithelial Cells In Vitro
WebJan 1, 2013 · Suspend the ciliary pellet in 500 μl of ice-cold axoneme buffer (20 m M potassium acetate, 5 m M MgSO 4, 0.5 m M EDTA, 20 m M HEPES, pH 7.6). 7. To obtain axonemes, suspend cilia in 500 μl of ice-cold motility buffer (5 m M MgSO 4, 1 m M EGTA, 30 m M HEPES, 1% PEG, pH 7.6). WebJan 1, 2013 · PHEM buffer: 25 mM HEPES, 10 mM EGTA, 60 mM PIPES, 2 mM MgCl 2. Add in the given order and adjust pH to 6.9 with 10 M KOH. Filter-sterilize and store at 4 °C. 3. 4 % paraformaldehyde (PFA): Dissolve 4 g PFA in 20 mL 0.1 M NaOH by heating it in a 80 °C water bath for 2–3 min. Cool down to room temperature and add 80 mL PHEM buffer (see … chocolate cream cheese icing with cocoa
Discovery and Functional Evaluation of Ciliary Proteins
WebDec 21, 2024 · The fixative was in PHEM buffer (5 mM HEPES, 60 mM PIPES, 10 mM EGTA, 2 mM MgCl2) because it provides better preservation of the overall cell structure than … WebMar 31, 2016 · PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, … http://www.emanuelelab.com/reagents-solutions-etc/ chocolate cream cheese icing using cocoa