The expression of OsEGL1 is significantly increased in rice seedling leaves by methyl jasmonate, abscisic acid, ethephon, and mechanical wounding treatments, suggesting that OsEGL1 may act in cell wall loosening and elongation by hydrolyzing (1,3;1,4)-β-glucan in the cell wall matrix on … See more The wild-type rice (Oryza sativa) used in this study was a japonica rice variety, Hejiang 19. The transgenic plants and wild-type plants were grown in an experimental field at Wuhan University Institute of Genetics … See more The maize (Zea mays) ubiquitin 1 (Ubi1) promoter and Nos terminator cassette were inserted into the HindIII and EcoRI sites of pCAMBIA 1301, resulting in plasmid pCU (Chen et al. 2007). To make a dsRNAi construct, … See more Total RNA was extracted using TRIzol reagent (Gibco BRL) according to the manufacturer’s protocol from various tissues of both wild … See more Total RNA was separated on a denaturing 1.5% formaldehyde agarose gel (Sambrook et al. 1989). Loading of equal amounts of RNA was confirmed by ethidium bromide staining. The RNA was transferred to … See more Webthe wounding-induced rice endo-(1,3;1,4)- -glucanase OsEGL1, suggesting that both enzymes may act in concert in remodeling of damaged cell wall. Among oligosaccharides tested, Os4BGlu12 hydrolyzed 1-(1,4)-linked glucooligosaccharides with highest catalytic efficiency (k cat/K m =2.7–4.9s− mM−1) when the degree of polymerization ranged ...
A rice -1,3-glucanase gene Osg1 is required for …
WebMay 1, 2000 · Akiyama et al. (2009) reported that the increase in the leaf elongation rate of rice seedlings treated under mechanical wounding was concomitant with the increase expression levels of OsEGL1, an ... WebMar 21, 2024 · OBSL1 (Obscurin Like Cytoskeletal Adaptor 1) is a Protein Coding gene. Diseases associated with OBSL1 include Three M Syndrome 2 and Three M Syndrome … galaxy a53 5g headphone
Expression of an endo-(1,3;1,4)-β-glucanase in response to …
WebNov 1, 2009 · The OsEGL1 expression levels were increased after 6-d imbibition and remained high at least until day 14 (Figure 3 a). In 10-d-old rice seedlings, OsEGL1 … WebRice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides WebWe isolated two rice endo-(1,3;1,4)-β-glucanase genes, denoted OsEGL1 and OsEGL2, which encoded proteins that shared 64% amino acid sequence identity. Both the OsEGL1 and OsEGL2 genes were successfully expressed in Escherichia coli to produce functional proteins. Purified OsEGL1 and OsEGL2 proteins hydrolyzed (1,3;1,4)-β-glucans, but not … galaxy a53 5g ohio state case