Human dutpase
WebMar 12, 2024 · Human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase), essential for DNA integrity, acts as a survival factor for tumor cells and is a target for … WebAug 7, 2003 · A high cellular dTTP: dUTP ratio is essential to avoid uracil incorporation into DNA, which would lead to strand breaks and cell death. We report the first detailed atomic-resolution structure of a eukaryotic dUTPase, human dUTPase, and complexes with the uracil-containing deoxyribonucleotides, dUMP, dUDP and dUTP.
Human dutpase
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WebAug 1, 1997 · Expression of dUTPase isoforms during serum stimulation and serum starvation of 34Lu human lung fibroblasts. A, 34Lu cells were driven into G 0 by serum starvation for 72 h. WebWe previously performed a computational sequence analysis of the 5′ region of the human dUTPase gene and identified putative binding sites for NFκ-B, Sp1 and E2F (Supplementary Figure 1A). The presence of Sp1- and E2F-binding sites is consistent with the hypothesis that the dUTPase promoter is regulated in a cell-cycle-dependent manner ( 31 ).
WebA fragment encoding human dUTPase (GenBank accession no. U62891) was amplified from reverse-transcribed placental RNA (cDNA),purified,andclonedintothepCAL-n-EKvector.Positives were analyzed by DNA sequencing. Human dUTPase (CBP-fusion protein) was purified and assayed for dUTPase activity (see below). HPLC Analysis of …
WebApr 30, 1999 · Superimposition of subunit structures of the human dUTPase and HERV-K enzyme using the hairpin loop as point of origin (Figure 4) shows the two structures share very similar topology. However, a single α-helix shows considerable displacement in the HERV-K dUTPase and results in an opening up of the dUTP binding site in the HERV-K … WebIn previous work, this enzyme has shown good potential to degrade phosphotriesters, and based on this characteristic, we have applied the human dUTPase to the OP …
WebJan 28, 2024 · The human herpesviruses dUTPase proteins act as Pathogen-Associated Molecular Pattern (PAMP) molecules and, through their ligation with Toll-like receptor (TLR)2 homodimers in the case of the EBV dUTPase or TLR2/1 heterodimers in the case of the HSV-2, HHV-6, HHV-8 and VZV dUTPases, differentially activate NF-κB, with …
WebMar 22, 2024 · To analyze the substrate specificity of Dr-dUTPase, enzyme assays were performed using dATP, dTTP, dCTP, and dGTP as substrates at a final concentration of 100 µM and 4–6 µM dUTPase (Table 1).The K m obtained for the tested nucleotides ranged between 94 and 210 (Table 1).However, the performance of Dr-dUTPase for nucleotides … toy shock pinballWebIn previous work, this enzyme has shown good potential to degrade phosphotriesters, and based on this characteristic, we have applied the human dUTPase to the OP degradation. Molecular docking, chemometrics and mixed quantum and molecular mechanics calculations have been employed, showing a good interaction between dUTPase and OP. toy shock digital pinballWebA recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 70-252 of Human dUTPase. Source: E.coli Amino Acid Sequence: MGSSHHHHHH … toy shock haunted houseWebSep 21, 2011 · Function. Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and … toy shitz puppy for sale in floridaWebDec 1, 2007 · Human dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA. The nuclear isoform of the enzyme has been proposed as a promising ... toy shiz tmntWebA recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 70-252 of Human dUTPase. Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI … toy shocksWebInhibition of human deoxyuridine triphosphatase (dUTPase) has been identified as a promising approach to enhance the efficacy of 5-fluorouracil (5-FU)-based chemotherapy. This study describes the development of a novel class of dUTPase inhibitors based on the structure–activity relationship (SAR) studies of uracil derivatives. Starting from the weak … toy shock mini pinball