Glut1 hif
WebJul 20, 2009 · The relative expression ratio of GLUT1 mRNA in hypoxic conditions was analyzed by RT-PCR. Ten cancerous tissues collected from human colorectal cancer patients were examined. HIF-1α and HIF-2α levels were measured to indicate the degree of hypoxia, and gene expression under hypoxic conditions was determined. WebJan 24, 2024 · To validate Glut1, HK2, PFKL, and LDHA as HIF-1 targets, we knocked down HIF-1α (siHIF-1α) and HIF-2α (siHIF-2α) in macrophages (Fig. 4 i–l). Except HK2, all genes increased under hypoxia in control and HIF-2α knockdown cells but the expression was significantly reduced when HIF-1α was knocked down.
Glut1 hif
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WebSep 19, 2013 · The mechanisms underlying this increase are not completely elucidated in the present study, but the possibility exists that TAC induced the activation of the MHC‐rtTA transgene in G1HA mice, which might have contributed to the further induction of the GLUT1 transgene. Hif‐1 α, a transcription factor known to induce GLUT1 expression, was ... WebNov 18, 2013 · To reconfirm the importance of HIF to tumor growth in a xenograft model, the expression of HIF1β protein in 786-O cells was stably suppressed by an shRNA construct HIF1b-1770 . Suppression of HIF1β’s expression in 786-O cells also eliminated the expression of GLUT1, a marker of HIF’s transcriptional activity (Figure S2 in File S1).
WebSep 22, 2024 · HIF-1α does not induce GLUT1 translocation. A number of genes that are induced under hypoxia may also have a role in GLUT1 translocation. Hypoxia inducible factor-1 (HIF-1) is an important regulator of the metabolic adaptation of cells to hypoxia. CoCl 2 stabilizes HIF-1α and mimics the hypoxic environment in cells . WebCarbohydrate metabolism and mediators of metabolic reprogramming. Cancer cells must acquire a greater amount of nutrients, especially glucose. One of the mediators is HIF-1, which increases glucose uptake through the induction of GLUT-1, GLUT-4 and GLUT-1; simultaneosly, it can also be stimulated by TGF-β through the PI3K/AKT/mTOR pathway.
WebMay 2, 2012 · Glut-1, HIF-1α, PI3K and p-Akt expression in neoplastic cells may be important in the development of a ceruminous adenoma. In conclusion, ceruminous adenoma of the EAC is rare type of tumour that is diagnosed histologically. The treatment of choice is wide local excision with clear margins. Glut-1, HIF-1α, PI3K and p-Akt … WebJan 19, 2024 · There was a significantly decreased HIF-1α and GLUT-1 after HIF-1α and GLUT-1 double gene knockout compared with before HIF-1α and GLUT-1 double gene knockout (P<0.001, respectively; Figure 4). The GLUT-1 and HIF-1α gene double knockout model in HEp-2 cells may contribute to functional analyses of these markers of cellular …
WebFeb 13, 2024 · If the protein expression of c-myc, HIF-1α, and glut1 is inhibited, the synthesis of ATP in the cell will be reduced, so these three proteins are identified as the key proteins of energy production. This study is the first to demonstrate that ARV σA protein can promote ATP production in cancer cells to facilitate viral replication.
WebMay 18, 2016 · We found that the expression of Pdk1 gene was more rapidly induced in hypoxia than iNOS or Glut1. The accumulation of HIF-1α at the HRE region was not altered, whereas the mRNA turnover kinetics ... thornaby place stockton on teesWebThe present study was designed to test whether HIF-1alpha is involved in hypoxia-induced activation of GLUT1 expression using trophoblast-derived human BeWo and rat Rcho-1 … thornaby pool swimming timetableWebFeb 29, 2016 · Studies have proposed that the HIF-1α upregulates Glut1, 37 and HIF-1α contributes to HSPC homeostasis. 18,20 We first assessed the hypoxic state of the BM cells isolated from irradiated ApoE −/− … thornaby pavilion gym opening timesWebJun 16, 2024 · Therefore, the main aims of this study were to: (1) verify that GLUT1 is a potential target gene of miR-34a; (2) analyze the effect of GLUT1- knockdown on the HIF-1a and p53 signaling pathway in GIFT liver cells; and (3) investigate how inhibition of miR-34a expression affects the response of GIFT to hypoxia stress. thornaby pavilion leisure centreWebAug 8, 2013 · Hypoxia inducible factor-1 alpha (HIF-1α), induced by tumor hypoxia, regulates tumor cell metabolism and metastasis by up-regulation of c-Met, carbonic anhydrase 9 (CA9) and glucose transporter 1 (GLUT1). The prognostic significance of hypoxia and metabolic markers is not clearly defined in cervical cancer. Here, we have … thornaby road stocktonWebApr 13, 2024 · GLUT-1 and HIF protein co-expression in the CDH lung. Confocal microscopic images show an increase in GLUT-1 (red), HIF-1α (green), and DAPI (blue) expression by immunofluorescence in the terminal airway of the NCDH lungs compared with NC and VC. However only HIF-1α is expressed in the lung periphery, with increased … umich ann arbor csWebJun 16, 2024 · Therefore, the main aims of this study were to: (1) verify that GLUT1 is a potential target gene of miR-34a; (2) analyze the effect of GLUT1- knockdown on the HIF … thornaby primary school stockton