Ehrlichia p28-specific antigens
WebFeb 1, 2024 · Ehrlichia canis is a common tick-borne intracellular pathogen causing canine monocytic ehrlichiosis (CME) in dogs worldwide. The aims of this study were to investigate the genetic diversity and antigenicity of E. canis based on the p28 and trp36 genes in dogs in Thailand. The E. canis p28 and trp36 genes were amplified by the polymerase chain … WebPCR was used to amplify a 537-bp region of an Ehrlichia ewingii gene encoding a homologue of the 28-kDa major antigenic protein (P28) of Ehrlichia chaffeensis. The E. ewingii p28 gene homologue was amplified from DNA extracted from whole blood obtained from four humans and one canine with confirmed …
Ehrlichia p28-specific antigens
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WebImmunoreactive proteins of Ehrlichia canis and Ehrlichia chaffeensis that have been characterized include a family of 28-kDa major outer membrane proteins (p28) and two large antigenically divergent surface glycoprotein orthologs. WebJan 1, 2015 · Ehrlichia-specific IgM or IgG or both antibodies can be detected using slides ... Unique macrophage and tick cell-specific protein expression from the p28/p30-outer membrane protein multigene locus ... Molecular variation in the variable-length PCR target and 120-kilodalton antigen genes of Ehrlichia chaffeensis from white-tailed deer ...
WebEhrlichiae are obligately intracellular bacteria that reside and replicate in phagocytes by circumventing host cell defenses and modulating cellular processes, including host cell gene... WebPrimers were selected from the E. ewingii p28 sequence and used to develop a species-specific PCR diagnostic assay. The p28 PCR assay amplified the expected 215-bp …
WebEhrlichia -specific IgG antibody responses develop in mice immunized with the P28 outer membrane proteins. Ehrlichia muris specific IgG antibody titers in all vaccinated groups … WebJul 1, 2024 · The efficacy of antibody detection tools for all stages of Ehrlichia canis infections and for various genotypes remains unclear. We produced recombinant gp36 (rgp36) antigens from different isolates of Thai E. canis to confirm the immunoreactivities to these recombinant proteins from naturally infected dogs. Sera and blood samples were …
WebMice immunized with P28-9, P28-12, P28-19, or the mixture induced Ehrlichia-specific CD4 + Th1 cells. Interestingly, mice immunized with P28-14, orthologs of which in E. …
Webresponses to Ehrlichia chaffeensis from E. canis, we also included peptides from OMPs expressed in E. chaffeensis– infected macrophages (P28-19) and in infected tick cells (P28-14)5 to determine the cross-reactivity of E. canis and A. platys antibodies in serum to these peptides of E. chaffeensis. The OMP-1X gene for A. platys was chosen ... can a 2006 ford ranger be flat towedWebPartial protection induced by immunization with P28-9, P28-12, and P28-19 against Ehrlichia was associated with the generation of Ehrlichia-specific cell-mediated and humoral immune responses. AB - The obligately intracellular bacterium Ehrlichia chaffeensis that resides in mononuclear phagocytes is the etiologic agent of human … fish a week 週一魚WebWe previously reported on the identification of two conserved major immunoreactive antigens, gp36 and gp19, which are the first proteins to elicit an E. canis-specific antibody response, and gp200 and p28, which elicit strong antibody responses later in the acute phase of the infection. fishaw electric bikeWebDec 5, 2013 · Several antigens of Ehrlichia spp. have been identified based on their reactivity with immune sera from infected hosts that include the major outer membrane … fishawellnessWebThe presence of Ehrlichia-specific antibodies as determined by IFA of sera from mice immunized with P28-14 suggests either possible cross-reactivity with the other P28s or … fisha wellness centreWebDec 1, 2011 · Mice immunized with P28-9, P28-12, P28-19, or the mixture induced Ehrlichia- specific CD4 + Th1 cells. Interestingly, mice immunized with P28-14, orthologs of which in E. chaffeensis and E. canis are primarily expressed in tick cells, failed to lower the ehrlichial burden in the spleen. fish a weekWebA gene encoding a 28-kDa protein of Ehrlichia canis was cloned, sequenced, and expressed, and a comparative molecular analysis with homologous genes of E. canis, Cowdria ruminantium, and Ehrlichia chaffeensis was performed. The complete gene has an 834-bp open reading frame encoding a protein of 278 … can a 2004 jeep liberty be flat towed