Bv2 transfection
WebJan 10, 2024 · Cell transfection. BV2 cells were cultured in an incubator at 37 ℃ and 5% CO 2. MiR-200c-3p mimics, RIP2 OE (RIP2 CDS clone) or RIP2 siRNA was used to … WebBV2 cells were transfected with anti-miR-137-3p/NC and siNeuroD1 or not, then followed by simultaneous treatment with LPS and curcumin. (A) Levels of NeuroD1 were measured …
Bv2 transfection
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WebElectroporation is a physical transfection method that permeabilizes the cell membrane by applying an electrical pulse and moves molecules via the electrical field into the cell. It is a powerful tool for transfecting large DNA … WebApr 9, 2024 · BV2 mouse microglia with the Trem2 H157Y variant were generated using CRISPR-Cas9 technology. ... Trem2 was mutated by co-transfection of plasmids containing the gRNA and Cas9. The transfected BV2 microglia were plated in 96-well plates by limit dilution to generate isogenic single clones. The clones were selected from wells and …
WebOct 3, 2024 · BV2 cells (4×10 5 cells per well) were seed onto 6-well plates overnight to reach 70% confluence for transfection. Then, miR-340 mimic or miR-34 inhibitor was transfected into cells for 24 hrs using … WebApr 22, 2024 · BV-2 cells were transfected with 4 μg pCMV3-N-FLAG-SOCS3 and pCMV3-N-Myc-CSN3 plasmids for 48 h. Cell lysates were incubated with an anti-Flag antibody overnight at 4°C, and then incubated with protein A/G-agarose beads for 4 h at 4°C.
WebOct 6, 2024 · BV-2 cells were transfected with either 2R or 66R plasmid as described previously. Twenty-four or 48 h after transfection, cells were washed twice with ice-cold DPBS and scraped in 200 μL ice-cold DPBS. Samples were stored at −80°C until total RNA was extracted (11828665001; Roche Molecular Systems). WebBV2 cells infected with B. suis s2 were transfected with PPM1A shRNA or control shRNA. Cells were collected for western blot analysis. The blots shown are representative of …
WebJan 1, 2024 · Transfection of BV2 cells with Glial-Mag, Lipofectamine 2000 and X-tremeGENE with eGFP plasmid DNA induced a modest secretion of the pro …
WebMay 1, 2005 · and BV2 were plated in 96-well plates (Primaria, BD ... has recently emerged as a specific and efficient method to silence gene … themen referat umweltWebTwo groups of lentiviruses were transfected into BV2 cells and screened with puromycin. Fluorescence microscopy and flow cytometry were used to observe the expression of … themen resilienzWebMaterials and methods: BV2 cells were pretreated by RSV and stimulated with lipopolysaccharide (LPS). Cell Viability was checked using a MTT assay. Real-Time PCR was performed to detect the levels of pro-inflammatory cytokines (tumor necrosisfactor-α－TNF-α, interleukin-1β－IL-1β and interleukin-6 － IL-6) and miR-146a-5p expression. tiger eye meaning and healing propertiesWebElectroporation is a physical transfection method that permeabilizes the cell membrane by applying an electrical pulse and moves molecules via the electrical field into the cell. It is a powerful tool for transfecting large DNA … tiger eye storage cheraw scWebAltoFect™ Transfection Kit includes Transfection Enhancer reagent and recommended transfection protocol. Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid. Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems. AltoFect reagent is designed for hard-to-transfect cell lines and primary ... tiger eye metaphysical properties and meaningWebJan 11, 2024 · Background Microglial activation is a prominent feature of neuroinflammation, which is present in almost all neurodegenerative diseases. While an initial inflammatory response mediated by microglia is considered to be protective, excessive pro-inflammatory response of microglia contributes to the pathogenesis of neurodegeneration. Although … themen religion referatWebJan 1, 2024 · The BV2 cell line is a murine cell line of microglial origin which is often used to study this cell type in vitro. Unfortunately, this microglial cell line is, like other phagocytic … themenrestaurant